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federal_dot_testingDOT Drug Testing Requirements

The Department of Transportation outlines strict drug testing requirements for anyone executing a "safety sensitive" position within the transportation industry.

Some of the requirements include strict regulations regarding:

  • Urine sample collection (specimen collectors, collection sites, detailed procedures)
  • Breath alcohol collection procedures
  • Laboratory procedures (forms, equipment, supplies to use for urine collection)
  • Reporting (medical review officers and verification process)
  • Record keeping of final results (confidentiality and release of information)


Be sure to read all regulations outlined in the DOT's rule 49 CFR Part 40.

Testing Procedures

Federal Motor Carrier Safety Administration

If your organization is regulated by the FMCSA, you must comply with the detailed Federal Motor Carrier Safety Administration (FMCSA) Requirements

Federal Transit Administration

The primary goal of the FTA is to develop new transit systems and improve and maintain existing systems, however they do have detailed Federal Transit Administration (FTA) Requirements for drug testing

Federal Aviation Administration

The Federal Aviation Administraion (FAA) regulates all aspects of the country's aerospace system, including safety requirements for all personnel and includes detailed Drug Testing Requirements specific to the FAA.

Federal Railroad Administration

The Federal Railroad Administration (FRA) provides safe, reliable rail transportation, and in order to provide that the FRA requires a strict (FRA) Drug Testing Policy

Coast Guard

The United States Coast Guard (USCG) enforces maritime law and ensures maritime safety, security and stewardship of people on the water. Because of this, they have detailed (USCG) Drug Testing Requirements.

Pipeline & Hazardous Materials Safety Administration

Working on our country's pipelines can be very risky business. A lot is at stake and drug-free workers are critical to pipeline integrity, which is why they have in place a detailed (PHMSA) Drug Testing Policy

DOT Alcohol Testing Requirements

Detailed DOT Alcohol Testing Requirements

Transmetron - A Qualified DOT-drug Testing Facility

Transmetron Drug Testing is a full-service Third Party Provider (TPP) for DOT urine collections for drug testing. We can either collect the specimen(s) on-site in our facility, or our mobile team can perform the collection(s) on-site.

We follow procedures outline by the Department of Health and Human Services (DHHS). Only labs qualified by SAMHSA may be used for DOT drug testing. We ensure that each step we take safeguards the accuracy of the testing being performed and protects both drivers and passengers alike.

What are the testing procedures

The official drug testing procedures are based on those established by the Department of Health and Human Services. These procedures include: urine sample collection, laboratory procedures, and reporting and record keeping of final results. Only laboratories qualified by Health and Human Services under the National Laboratory Certification Program (NLCP) may be used. These procedures exist to safeguard accuracy and to protect the privacy of drivers.

The Omnibus Transportation Employee Testing Act of 1991 requires drug and alcohol testing of safety-sensitive transportation employees in aviation, trucking, railroads, mass transit, pipelines and other transportation industries.

Transmetron offers fully DOT-compliant drug testing services as established by 49 CFR Part 40.

We also offer the following Federal DOT compliant services in addition to our Drug Testing Services:


Please contact Transmetron for more D.O.T. Testing information.

DOT Mobile Drug Testing